时间分辨免疫荧光法用于梅毒抗体测定的探讨

The discussion of Time-resolved immunofluorescence method for determination of syphius antibody

目的通过对时间分辨免疫荧光法(TRFIA),酶联免疫吸附试验(ELISA),明胶颗粒凝集试验(TPPA)三种方法测定梅毒特异性抗体(TP)的比较,并对TRFIA法的特异性、灵敏度、线性进行方法学评价,探讨临床常规使用TRFIA方法检测梅毒特异性抗体的可行性。方法对2012年1月至2012年7月我院256名门诊患者标本分别用TRFIA、ELISA、TPPA三种方法测定TP,并对TRFIA检测系统的灵敏度、批内,批间精密度、线性范围进行测定。结果TRFIA与TPPA法检测相比其灵敏度为100%,特异性为98.2%。TP阴阳性结果,经配对卡方检验P>0.05,三种方法无统计学差别。最低检测浓度0.15NCU,相关系数分别为0.95、0.999,高、中、低三种浓度血清的批内、批间精密度CV<10%。结论 TRFIA法测定TP灵敏度、特异性、方法性能、相关性均好,可作为临床TP常规筛查方法。

Objective Evaluate TRFIA for the detection of a syphilis specific antibody.Methods The sensitivity,specificity,precision,linearity,the minimum detectable concentration of TRFIA method were test,and in the test of sensitivity and specificity do TPPA of syphilis antibody determination,with TPPA as the gold standard.Results The TRFIA is sensitivity of 100％,specificity of 98.2％,the detectable concentration of 0.15NCU.Conclusion TRFIA is sensitive,specific,high-performance,and good consistency with TPPA,so TRFIA can be used as a routine screening method for syphilis.