响应面优化酶法提取紫菜多糖工艺研究

Response surface methodology for optimization of enzymatic extraction of polysaccharide from Laver

利用纤维素酶辅助提取紫菜多糖,以酶添加量、提取温度、提取时间和pH作为响应面设计的变量。结果表明,纤维素酶辅助提取紫菜多糖的优化工艺条件为:酶添加量1.5%,提取温度51℃,pH 5.0,提取时间为80 min,在此条件下,多糖得率为19.46%。

Using cellulase assisted extraction of Laver polysaccharide,with the enzyme concentration,temperature,hydrolysis time and pH as a response surface design variables.The result showed that the optimum hydrolysis conditions for a considerably enhanced extraction yield of polysaccharide were determined as follows：1.5％ of enzyme concentration,51 ℃,pH 5.0 and 80 min of extraction time.Under this condition,the polysaccharide yield is 19.46％.