一株猪丹毒杆菌的分离鉴定及其对活疫苗免疫猪的攻毒试验

Identification of a Swine Erysipelas Bacillus Strain and Challenge Tests for Swines Immunicated with Living Vaccines

从广东清远某猪场发病母猪中分离到一株QY菌株,通过对其培养特性、形体特征、生化反应、动物回归等试验,确定其为猪丹毒杆菌。采用国内常用GC42株、G4T10株猪丹毒弱毒疫苗分别免疫断奶后2个月龄猪,用QY株、标准猪丹毒强毒菌株培养液攻毒。结果表明,用QY株攻击免疫猪,猪丹毒弱毒疫苗GC42株和G4T10株的免疫猪均保护5/5,对照猪5/5发病且2/5死亡;用标准猪丹毒强毒菌株攻毒,猪丹毒弱毒疫苗GC42株和G4T10株的免疫猪均保护5/5,对照猪5/5发病且3/5死亡。

A bacillus was isolated from an infected sow in a pig farm in Qingyuan, Guangdong.The isolate,named QY, was determined to be Erysipelothrix rhusiopathiae by the cultural characteristicS, the shape characteristics, biochemical reactions and animal regression test. Pigs of two months old after weaning were immunized with attenuated vaccine strains （the common domestic strains GC42 and G4T10 respectively） and then challenged with standard virulent strain.The results suggested that the attenuated vaccine immunized group was fully protected after challengeed with QY strain, the control group had a mobidity of 5/5 and mortality of 2/5 ; after challenged with standard virulent strain, the attenuated vaccine immunized group was fully protected, the control group was with a mobidity of and mortality of 3/5.