摘要
目的:探讨PJA1在宫颈癌Hela细胞中的促凋亡作用。方法:采用流式细胞仪检测转染PJA1后的宫颈癌细胞12,24,36,48 h的凋亡变化。采用Western免疫印迹(Western Blot)检测凋亡通路。结果:宫颈癌Hela细胞在转染PJA1后12 h凋亡率最低,48 h最高,并且在凋亡中与caspase通路相关。结论:PJA1可以通过caspase通路促进宫颈癌Hela细胞凋亡。
Objective :To investigate the apoptosis characteristics of PJA1 cervical cancer Hela. Methods :The apoptosis of cervical cancer Hela were observed after being transfection PJA1 for 12 h,24 h,36 h and 48 h by flow cytometry. And using Western blot detecting apoptotic pathways. Results:The lowest rate of apoptosis of cervical cancer Hela was found 12 hours, and the highest rate of apoptosis observed at 48 h. In the apoptosis associated with caspase pathway. Conclusion:PJA1 can promote cervical cancer Hela apoptosis by caspase pathway.
出处
《临床医药实践》
2014年第3期202-204,共3页
Proceeding of Clinical Medicine