金钗石斛对糖尿病肾病大鼠肾组织NF-κB、IL-6表达的影响

Effects of the aqueous extract of dendrobium nobile lindl on NF-κB and IL-6 expression of renal tissue in diabetic rat model

目的观察金钗石斛(DNL)水提物对糖尿病大鼠肾组织核转录因子-κB(NF-κB)、白细胞介素-6(IL-6)表达的影响。方法采用链脲佐菌素建立糖尿病大鼠模型,分为健康对照组、糖尿病组、金钗石斛干预1组(DNL1组)、金钗石斛干预2组(DNL2组),灌胃给药12周。分别测定血糖、血尿素氮、血肌酐、尿肌酐、24h尿蛋白,并计算肌酐清除率,蛋白质印迹法检测NF-κB、IL-6蛋白表达水平,实时荧光定量PCR检测IL-6mRNA的表达水平。结果与健康对照组比较,12周末糖尿病组大鼠血糖、尿素氮、24小时尿蛋白显著增高(P<0.01),肌酐清除率显著降低(P<0.01),NF-κB、IL-6蛋白表达水平与IL-6mRNA水平明显增高(P<0.05);与糖尿病组比较,DNL1组、DNL2组大鼠血糖、尿素氮、24h蛋白尿明显降低(P<0.05),肌酐清除率明显增加(P<0.05),NF-κB、IL-6蛋白表达水平与IL-6mRNA水平明显降低(P<0.05)。结论 DNL能抑制糖尿病大鼠肾组织中NF-κB的活化,进而降低IL-6的表达,对糖尿病组大鼠肾脏起到保护作用。

Objective To observe the effects of dendrobium nobile Lindl（DNL） on Nuclear factor（NF）-κB and interleukin（IL）-6 expression of renal tissue in diabetic rat model .Methods The diabetes mellitus rat model was induced by streptozotocin .All rats were divided into four groups at random ：the normal control group（group N） ,diabetic control group（group diabetes mellitus） ,drug group one（group DNL1） ,drug group two（group DNL2） ,and administrated for 12 weeks .The levels of the fasting plasma glucose , urea nitrogen ,serum creatinine ,urine creatinine ,24 hours urinary protein were determined .NF-κB and IL-6 protein expression levels were measured by Western-Blot .IL-6 mRNA expression levels were measured by Real-time fluorescence quantitative PCR . Results Compared with group N ,the blood sugar ,urea nitrogen ,24 hours urinary protein of group diabetes mellitus were signifi-cantly higher（P＆lt;0 .01） ,creatinine clearance was significantly reduced （P＆lt;0 .01） ,the NF-κB、IL-6 protein expression levels and IL-6 mRNA level increased significantly （all P＆lt;0 .05） .Compared with group diabetes mellitus ,the blood sugar ,urea nitrogen ,24 hours urinary protein of group DNL1 and group DNL2 were significantly decreased（P＆lt;0 .05） ,creatinine clearance increased significantly （P＆lt;0 .05） ,the NF-κB、IL-6 protein expression levels and IL-6 mRNA level decreased significantly（all P＆lt;0 .05） .Conclusion The DNL could protect the kidney of diabetic rats ,reduce the levels of NF-κB 、IL-6 protein and IL-6 mRNA expression in renal tissue of diabetic rats .