H_3BO_3及植物生长调节剂对锥栗花粉萌发率的影响

Effects of H_3BO_3 and plant growth regulators on pollen germination rate in Castanea henryi

为了研究锥栗花粉萌发的最适条件,以白露仔花粉为试材,采用花粉离体培养法,探索了H3BO3和2,4-D、GA3、IBA、NAA及其组合对锥栗花粉萌发率的影响。结果表明:(1)在单因素处理试验中,H3BO3与这4种植物生长调节剂都能促进锥栗花粉萌发,其中以H3BO3、2,4-D、GA3、IBA的促进作用较为明显,最适浓度分别为100、1.0、60.0、2.0 mg·L-1,萌发率分别达到了41.75%、40.66%、38.73%、43.15%(CK的萌发率为18.28%);(2)在正交试验中,4种因子的配比能显著提高锥栗花粉萌发率,最佳配比为:100 mg·L-1 H3BO3+2.0 mg·L-1 2,4-D+70.0 mg·L-1 GA3+2.0 mg·L-1 IBA,其萌发率达到62.34%,比对照提高了44.06%。

In order to screen out the optimal condition for pollen germination in Castanea henryi, taking pollens in C. henryi ＇Bailuzi＇ materials, the effects of H3BO3 and some plant growth regulators, such as, 2,4-dichlorophenoxyacetic acid （2,4-D）, gibberellic acid （GA3）, indolebutyric acid （IBA） and naphthalene acetic acid （NAA）, on pollen germination rate were studied by using in vitro tissue culture method. The results indicate that H3BO3 and the four plant growth regulators can promote pollen germinating in single factor treatment tests. The effects of H3BO3, 2,4-D, GA3 and IBA are significant, the optimal concentrations are 100, 1.0, 60.0, 2.0 mg·L^-1 respectively, and the germination rates are 41.75%, 40.66%, 38.73%, 43.15% respectively （the germination rate is 18.28% in CK）. Combination of four factors can significantly increase pollen germination in C. henryi, and the best combination is 100 mg·L^-1H3BO3, 2.0 mg·L^-1 2,4-D, 70.0 mg·L^-1 GA3 and 2.0 mg·L^-1 IBA. The pollen germination rate can reach 62.34% which is 44.06% higher than CK.