藏茵陈总萜酮致突变性及抗突变性研究

Studies on the safety and anti-mutagenicity of total terpene ketones form Swertia mussotii

目的：研究藏茵陈总萜酮在不同剂量下的致突变及抗突变作用。方法：Ames实验采用预培养法，微核实验采用连续灌胃给药10d的小鼠骨髓嗜多染红细胞微核计数法。结果：致突变实验中，藏茵陈总萜酮在〈2500μg／皿和〈40mg／kg剂量下，未诱发Ames实验各菌株回变菌落数和微核实验骨髓嗜多染红细胞微核率的增高。抗突变实验中，藏茵陈总萜酮在625—2500μg/皿浓度范围内，可使阳性剂环磷酰胺和丝裂霉素C所诱发的高回变菌落数出现明显降低；藏茵陈总萜酮在50～100mg／kg剂量范围，可使阳性剂40mg／kg环磷酰胺或2mg／kg丝裂霉素C所诱发的高微核率出现明显降低。结论：本实验条件下，藏茵陈总萜酮不存在基因突变和染色体畸变作用，且有显著的抗突变作用。

Objective： To study the mutagenic and anti - mutagenic activity of total terpene ketones from Swertia mussotii （TYKS） under different doses. Methods： The studies were conducted with Ames test of preceding incubate and micronucleus test in mouse which was administrated gavage once a day for a consecutive 10 d. Results ： In mutagenic test, TTKS at a dose lower than 2000 μg/plate induced neither remarkable nor dose - dependent increase mutagenicity of the four strains and at a dose lower than 100 mg/kg did not increase the micronucleus frequencies of polychromatic erythrocytes. In anti - mutagenic test, comparing with the positive groups of CP and MMC, TTKS at 625 ～ 2500 μg/plate could decrease the colony numbers in T_100 and T_102 strains ; TYKS at 50 - 100 mg/kg could significantly reduced the rate of micronucleus frequencies polychromatic erythrocytes in bone marrow of mice induced by CP and MMC as compared with the positive control. Conclusion ： Under our experimental conditions, TTKS has no effect of mutagenesis and processes significant anti - mutagenic properties.