摘要
目的观察康复1号提取物对小鼠成骨细胞MC3T3-E1细胞增殖的影响。方法将稀释成不同梯度浓度的康复1号提取物和成骨样细胞MC3T3-E1共同体外培养,采用MTT法检测细胞的增殖。结果康复1号提取物低浓度组(1:2000)对MC3T3-E1细胞增殖无影响(P>0.05);中浓度组(1:1000)对细胞增殖影响明显,增殖率48.1%(P<0.05);高浓度组(1:250)增殖率68.1%(P<0.01)。结论体外实验表明,康复1号能明显促进小鼠MC3T3-E1细胞的增殖。
Objective To observe the effect of rehabilitation No.1 herb extracts on the proliferation of MC3T3-E1 cells. Methods Osteoblast-like MC3T3-E1 cells were cultured in vitro with different concentrations of herb extracts of rehabilitation No.1. Cell proliferation was detected by using MTT method. Results Low concentration group(1:2000) of the rehabilitation No.1 herb extract had no effect on the proliferation of MC3T3-E1 cells; the proliferation rate was 48.1% in middle concentration group (1:1000)(P&lt;0.05); and the proliferation rate of high concentration group (1:250) was 68.1%(P&lt;0.01). Conclusion Rrehabilitation No.1 herb extracts can promote the proliferation of MC3T3-E1 cells in vitro.
出处
《浙江中西医结合杂志》
2014年第3期198-199,共2页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
浙江省中医药科学研究基金项目(No.2012ZB004)
浙江省医药卫生平台重点资助计划(No.2012ZDA007)
浙江省医学卫生科学研究基金项目(No.2010KYB133)