引进海岛棉种质的SSR遗传多样性分析

Genetic Diversity Analysis of the Sea Island Cotton Introduced Using SSR Markers

本文利用95对SSR分子标记多对来自于前俄罗斯的33分海岛棉，以及5份来自埃及，1份来自美国，1份来自阿尔巴尼亚，9份来自新疆，7份来自国内云南，江苏，等地共56份海岛棉进行遗传多样性分析，结果如下：95对SSR引物在56个海岛棉品种中共测出了384个等位基因，其中多态性等位基因296个，占77.1%。每对引物等位基因变幅是2~9个，平均为4.1个。基因型多样性指数（H）在0.035~2.931之间，平均为0.879。Shannon信息指数（I）在0.09~4.417之间，平均为1.363 。各指数的趋势一致。95对SSR引物的多态性信息含量PIC变幅为0.035~ 0. 926,平均为0.698。56份海岛棉品种两两间的相似系数,分布在0.585~0.952之间，主要集中在0.6~ 0.8之间，占整个数据的97%，平均遗传相似系数为0.7。从整体上来看，56份海岛棉的遗传相似系数较高.从聚类图上可以看出，以遗传距离为0.69为标准，56份海岛棉品种可分为4类。第1类主要是前苏联海岛棉为主的27份品种，其中来自埃及的吉扎77和来自 美国的派字棉以及中239都归到了这一类。第二类稍微复杂，但主要是以来自新疆的6份海岛棉和来自埃及的吉扎系列的4份为主。第3类包括8个品种，主要是来自于前苏联的6个品种和来自于新疆的巴3116和河北的冀B91-45.第4类只有一个来自于前苏联的L-8007，独自成一类。研究结果再次说明SSR 作为一种共显性标记,尤为适用于海岛棉及其亲本的系谱分析及鉴定。因而, SSR标记技术可以为海岛棉育种实践工作中的亲本选配,提供可靠的分子水平上的依据。

The genetic relationship and genetic diversity of 56 Sea-island cotton （Gossypium barbadense L.） were analyzed by 95 pairs of SSR markers. Among the 56 Sea-island cotton varieties,33 of which come from former USSR,1 from USA,1 from Albania,9 from Xinjiang and seven respectively come s from Yunnan, Jiangsu , and other places of China. Among the 95 pairs of polymorphic SSR primers 384 alleles were amplified in total ,and 296 were polymorphic, which accounted for 77.1% .2 t o 9 alleles could be produced from each pair of primers with average of 4.1.The gene diversity （H） was 0.035 to2.931 with average of 0.879. Shannon＇s information index（I）was 0.09 to 4.417with average of 1.363.The trend of each index is accordant. The value of allelic polymorphism information content （PIC） ranged from 0.035 to 0. 926,on an average of 0.698 per SSR marker. The genetic similarity of paired varieties ranged from 0.585 to 0.952,with average of 0.7,and the genetic similarity of 97% tested materials ranged from 0.6 to 0.8.which showed a high genetic similarity. The result of UPGMA cluster showed that four groups could be clearly clustered when genetic similarity coefficient was given as 0.69.The first ？ group contained 27 varieties . mainly from former USSR. But Giza 77 ,Paizi cotton and zhong 239 were assigned？ to this group.The second group was a little complicated and mostly included 6 varieties from Xingjiang and 4 ？ varieties from Egypt . The third group contained 8 varieties and 6 of them were from former USSR but 3116 from Xinjiang and JI B91-45 from Hebei.were also clustered in this group. The last group contained only one variety L-8007 from former USSR. Our result showed again as a kind of codominant markers ,SSR can be used for the pedigree analysis and identification of Sea-Island especially.Therefor SSR markers proved to be the work of Sea-Island cotton breeding practice of matching parents, to provide a reliable basis for the molecular level.