珊瑚菜居群遗传多样性的SRAP分析

Genetic diversity analysis of Glehnia littoralis(Apiaceae) revealed by SRAP

利用SRAP对伞形科单种属珊瑚菜7个野生居群和1个栽培居群进行了研究.结果表明：共筛选出8个引物组合,在珊瑚菜8个居群中共扩增出168条条带,其中多态性条带为118条,多态性比率为70．23％；平均每对引物扩增的多态性条带为14．75.各居群之间珊瑚菜遗传相似性系数范围为0．8306~0．9836,遗传距离范围为0．0165~0．1856.聚类分析表明,以相似性系数大于0．8并结合地理分布来看,所研究的野生珊瑚菜居群可以大体分为3类,辽宁大连的野生居群为一类,山东威海-山东青岛的居群为一类,而山东日照-广州深圳之间的为一类.

Sequence-related amplified polymorphism （SRAP）was used to analyze genetic relationships of eight popu-lations of Glehnia littoralis F.Schmidt ex Miquel in China.Among them,seven populations were wild plants and one was cultivar plant.With eight SRAP primer combinations,1 6 8 loci were identified.Among them,1 1 8 were polymor-phic and accounted for 70.23% of total amplified loci,showing a high polymorphism.The genetic similarity among these eight populations ranged from 0.830 6 to 0.983 6 and the genetic diversity was 0.0165-0.1856.Based on the results of cluster and principal coordinate analysis,the eight populations of G.littoralis were divided into three groups.The population from Dalian of Liaoling Province was confirmed as Group A,while populations from Shan-dong Province （excluding Rizhao）were clustered into Group B.Group C comprised populations from Rizhao of Shan-dong Province to Shenzhen,Guangzhou.