戊巴比妥钠和乌拉坦对乙酰胆碱量效曲线的影响

Effect of Pentobarbital Sodium and Urethane on dose-response Curve of Acetylcholine

目的:筛选一种适合的麻醉药,在不影响实验结果的前提下,尽量减轻动物的痛苦,保障动物福利。方法:将24只家兔随机分为未麻醉组(对照组)、戊巴比妥钠麻醉组、乌拉坦麻醉组。制备离体回肠标本,在离体肠管恒温灌流装置中,给予不同浓度的乙酰胆碱,观察肠管的收缩幅度。冲洗肠管后,用阿托品(1×10-6 mol/μL)进行阻断,然后再给予不同浓度的乙酰胆碱,用BL-420E描记肠管收缩幅度的变化,并绘制量效曲线。结果:与对照组相比,经乌拉坦麻醉的家兔,肠管收缩幅度明显降低(P<0.05),阿托品阻断后乙酰胆碱的量效曲线没有右移。经戊巴比妥钠麻醉的家兔实验结果与对照组的实验结果无显著差异。结论:观察离体肠管的收缩活动可以用戊巴比妥钠进行麻醉而不能用乌拉坦麻醉。

Objective：To screening a suitable anesthetic for the purpose of safeguarding animal welfare,in the case of without affecting the experimental results and minimizing animal suffering.Methods：24 healthy rabbits were randomly divided into three groups：control group,sodium pentobarbital anesthesia group and urethane anesthesia group.Isolated rabbit ileums were prepared and placed into the constant temperature perfusion device,then given different concentrations of acetylcholine and atropine （1×10-6mol/L）.Intestinal contraction amplitude were observed and dose-response curves were drawn with BL-420E.Results：Compared with control group,intestinal contraction amplitude was significantly reduced in urethane anesthesia group（P〈0.05）.Dose-response curve of acetylcholine did not shift to the right after atropine blocked.However,there is no significant difference in sodium pentobarbital anesthesia group compared with the control group.Conclusion：To observe the isolated intestinal contractile activity,rabbit can be anesthetized with pentobarbital sodium rather than urethane anesthesia.