黧蒴栲遗传多样性的AFLP分析

AFLP Analysis of Genetic Diversity of Castanopsis fissa Rehd.et Wils

遗传多样性分析为黧蒴栲优良品种的培育和利用提供技术依据。利用AFLP技术对39个黧蒴栲半同胞家系进行遗传多样性分析,结果显示利用8对引物共扩增出清晰条带761条,其中有多态性的条带有565条,多态率为74.14%,半同胞家系之间遗传多样性较高。黧蒴栲不同优树采集地之间遗传多样性较大,遗传多样性表现出较明显的地域性。各优树采集地内的遗传多样性表现不一致:湖南通道、福建明溪、广东平远优树采集地内遗传变异小;广西永福、融水、平果3地优树采集地内遗传变异较大,存在较大的遗传多样性;广西苍梧优树采集地内遗传变异最大。黧蒴栲出现优良半同胞家系的几率和遗传多样性密切相关,遗传多样性高的出现优良半同胞家系的几率大。出现优良家系的几率与家系所处的地理位置具有相关性,优良率与家系所处的纬度呈高度负相关,与经度为中度负相关。

The AFLP analysis of genetic diversity provided scientific basis for breeding and utilizing of the good varieties of Cnstanopsis aqssa Rehd.et Wils. The genetic relationships among 39 Castanopsis/Tssa Rehd.et Wils＇ s half-sib families were analyzed by AFLP. The results showed that 8 pairs of primers produced 761 distinctive bands in total, 565 bands were polymorphic and the polymorphic rate was 74.14%, there were high genetic diversity among the different half-sib families. There were various genetic diversities among the different plus tree collection sites, the genetic diversity showed obvious regional character. There were different genetic diversity performances in the different collection sites： Hunan Tongdao, Fujian Mingxi and Guangdong Pingyuans＇ samples had low genetic variation. There were high genetic variations and genetic diversities in Guangxi Yongfu, Rongshui and Pingguos＇ samples. Cangwu＇ s samples had the highest genetic variation. The appearing chance of the excellent half-sib families of Cnstanopsis fissa Rehd.et Wils was closely related to the genetic diversity, the higher genetic diversity were, the more the excellent half-sib families appeared. The appearing chance of the excellent half-sib families had close correlated with the half- sib families＇ geographical position. The appearing chance of the excellent half-sib families had high reversely correlated with latitude and medium reversely correlated with longitude.