东方百合不同培养方式鳞茎淀粉粒亚显微结构比较研究

Comparison of Different Culture Methods in Ultra-structure of Starch Granules of Oriental Hybrid Lily

为了研究东方百合鳞茎发育的机理问题,以东方百合‘索蚌’为试材,进行了不同培养方式下鳞茎淀粉含量、可溶性糖含量、蛋白质含量等生理指标及淀粉粒亚显微结构的比较研究。结果表明:商品鳞茎淀粉粒数量明显比扦插鳞茎、试管鳞茎多,含水量比扦插鳞茎、试管鳞茎少,这与淀粉含量测定结果一致;商品鳞茎蛋白质含量最低、试管鳞茎最高;商品鳞茎基部、中部、上部都有大小淀粉粒并存,扦插鳞茎及试管鳞茎淀粉颗粒大小较一致,差异不明显;无论是商品鳞茎、扦插鳞茎还是试管鳞茎,基部淀粉粒数量都明显比中部和上部多。

In order to study the mechanism of bulb development, the test material was oriental hybrid lily ＇Sorbonne＇. Comparison of different culture methods in ultra-structure of physiological indexes （such as starch content, soluble sugar content, protein content） and starch granules of oriental hybrid lily was studied. Results showed that： the marketable bulb of starch granules was obvious more than that in cuttage and in test tube in quantity. Water content in the marketable bulb was the least in the three. The result was consistent with the determination of starch content. Protein content in the marketable bulb was the lowest, which in test tube bulb was highest. The starch granules which were big and small were co-exist in marketable bulb, and that was the same size in cuttage or in test tube. The quantity of bulb of starch granules was obvious the most in the basal than that of the middle and the upper, whether it was marketable bulb, bulb in cuttage and in test tube.