5种红树根际土壤真菌和内生真菌的分离及抑菌活性的研究

The Antimicrobial Activity of 5 Kinds of Mangrove Fungi in Rhizosphere Soil and Endophytic Fungi

研究药用红树植物根际土壤真菌和内生真菌次级代谢产物的抑菌活性,旨在获得有良好抑菌活性的菌株,以期寻找新型抗菌物质。以6种敏感细菌为实验菌株,采用纸片扩散法,根据观察并测量抑菌圈直径大小结果,对其代谢产物抑菌活性研究分析。从桐花、秋茄、白莲、木榄、老鼠簕中共分离得到50株真菌,其中16株真菌次级代谢产物对至少1株实验菌株有抑菌活性,占总分离菌株的30.77%,且对金黄色葡萄球菌的拮抗率最高达32%。研究表明,HGC-MT3-A,HGC-LT3-A和HGC-QJ1-A,HGC-MT3-A、HGC-LT3-A、HGC-QJ1-A,HGC-TY3-A的发酵粗体物分别对对大肠杆菌,藤黄微球菌,白葡萄球菌,枯草芽孢杆菌有很强的抑制作用。HGC-QJ1-A、HGC-QY2-A、HGC-MT3-A、HGC-TY3-A、HGC-LT3-A和HGC-TT22-A1对金黄色葡萄球菌、藤黄微球菌、白葡萄球菌和枯草芽孢杆菌均有很强的杀菌效果;HGC-LT3-A、HGC-TT22-A1对白葡萄球菌的杀菌浓度均为25 mg/mL;HGC-MT3-A、HGC-TT22-A1对大肠杆菌的杀菌浓度分别低至为1.56 mg/mL和3.125 mg/mL。红树根际土壤真菌和内生真菌次级代谢产物中存在着丰富的天然活性物质,可为寻找新型抗菌物质提供资源。

In order to obtain the new antibacterial compounds from the marine fungi, the antibacterial activities on the secondary metabolites of the medicinal mangrove rhizosphere soil of symbiotic fungi and endophytic fungi were tested. The secondary metabolites of marine fungi were tested 6 sensitive bacteria by the disc diffusion method according to its antibacterial circle diameter. 50 strains of fungi were isolated from the mangrove plants, Aegiceras cornieulata, Kandelia eandel, white lotus, Bruguiera cylindrica and Acanthus ilieifolius. The secondary metabolites of 16 fungi showed antibacterial activity, 30.77% of the total fungi, and the highest antibacterial activity of Staphyloccocus aureus reached to 32%. Accoding to the study, the secondary metabolites of HGC-MT3-A, HGC-LT3-A and HGC-QJ^-A, HGC-MT3-A, HGC-LT3-A, HGC-QJ1-A, HGC-TY3-A showed strong antibacterial activity for E.coli, Mierococcus luteus, White staphylococcus and Bacillus subtilis respectively. The secondary metabolites of HGC-QJ1-A, HGC-QY2-A, HGC-MT3-A, HGC-TY3-A, HGC-LT3-A and HGC-TT22-Al showed strong sterilization effect respectively to Staphyloceocus aureus, Microcoecus luteus, White staphylococcus and Bacillus subtilis; the bactericidal concentration of HGC-LT3-A and HGC-TT22-A1 to White staphylococcus were 25 mg/mL; the bactericidalconcentration of HGC-MT3-A and HGC-TT22-A1 to E.eoli respectively was 1.56 mg/mL and 3.125 mg/mL. The secondary metabolites of symbiotic fungi and endophytic fungi from the medicinal mangrove plants and rhizosphere soil were important resources of novel antibiotic compounds.