毛泡桐二倍体及其同源四倍体的AFLP和MSAP分析

Analysis of diploid and its autotetraploid Paulownia tomentosa with AFLP and MSAP

分别用AFLP和MSAP分子标记技术,研究了毛泡桐二倍体及其同源四倍体幼苗DNA碱基序列及其甲基化的变化。结果表明,毛泡桐二倍体及其同源四倍体DNA碱基序列在AFLP水平上没有发生变化;在MSAP扩增电泳凝胶上,毛泡桐二倍体及其同源四倍体分别检测到2 262和2 180个扩增位点,DNA甲基化位点占总扩增位点的比例分别为35.32%和38.58%,其中DNA全甲基化比例则为12.86%和14.13%;毛泡桐同源四倍体DNA甲基化和去甲基化频率高于其二倍体,总DNA甲基化多态性低于二倍体。

DNA base sequence and DNA methylation of diploid and autotetraploid Paulownia tomentosa seedlings were investigated with amplified fragment length polymorphism （AFLP） and the methylation-sensitive amplification polymorphism （MSAP） respectively. The results show that there was no change of their DNA base sequences between diploid Paulownia tomentosa and its autotetraploid; there were 2 262 and 2 180 sites were detected in the diploid and its autotetraploid by MSAP, and their methylation sites were 35.32% and 38.58% （where fully methylation rate were 12.86% and 14.13%） respectively; DNA methylation and demethylation levels of the autotetraploid Paulownia tomentosa were higher than those of its corresponding diploid, but its total DNA polymorphism was low than the diploid.