基于[C_(10)-mim^+]Br^-固定HRP于Au/石墨烯复合材料电极表面的新型H_2O_2酶生物传感器

Novel H_2O_2 enzyme biosensor based[C_(10)-mim^+]Br^-fixed HRP on Au/graphene nanocomposite electrode

利用长链离子液体特殊的性质,用其固定HRP于Au/graphene电极表面(Nafion/HRP/[C10-mim+]Br-/Au/Gr/GCE)组装成H2O2传感器。用透射电镜来表征Au/氧化石墨烯的形貌,金纳米颗粒很均匀的分散在石墨烯表面,并不存在团聚现像。电化学技术检测Nafion/HRP/[C10-mim+]Br-/Au/Gr修饰电极对H2O2的响应情况,显示修饰电极对H2O2有很好的响应,在H2O2浓度2.0×10-6~1.2×10-3 mol/L的范围内,还原电流与浓度存在线性关系(R=0.997),检测限为3.0×10-7 mol/L;另外传感器具有很好的稳定性和选择性,为生物分子的检测提供新方法。

Novd nanocomposite materials modified electrode（Nafion/HRP/[C10-mim＋] Br/Au/Gr） was prepared by utilizing long chain ionic liquids immobilization HRP （horseradish peroxidase）on Au/graphene electrode surface. The morphologies of the Au/graphene oxide nanoeomposite film were investigated by transmission electron microscopy, the Au nanoparticles were uniformity dispersed on the graphene oxide surface and non-existent rally. Electrochemical response of H2O2 on the electrode was investigated by electrochemical technique including cyclic voltammetry and amperometry. The research results showed that the modified electrode exhibited excellent electrocatalytic effect. Under the optimal experimen- tal conditions, the reduction peak current on the electrode was proportional to the concentration of H2 O2 in the range from 2.0 × 10 -6 -1.2× 10-3 mol/L with a correlation coefficient of 0. 997. The detection limit was 3.0× 10-7 mol/L. Futhermore, the sensor had good stability, selectivity and long life time. The sensor provided new means for the determination of biomol- ecules.