摘要
以筛选得到的活性强并导入玉米赤霉烯酮降解酶基因的重组毕赤酵母ZLHY6为出发菌株,通过响应面法优化培养基。结果表明:经过对增殖培养基BMGY的优化,获得适合毕赤酵母ZLHY6生长的最优培养基配方:3.5%甘油、0.65%酵母提取物、10%磷酸钾缓冲液、1.25%硫酸铵、0.45%硫酸镁、0.04%硫酸钙。
The strong vitality strains, imported with zearalenone degrading enzyme gene, were selected as initial strains, and the medium was optimized by response surface analysis. The results indicated that the optimal medium suitable for Pichia ZLHY6 was 3.5% glycerin, 0.65% yeast extract, 10% buffer solu- tion of potassium phosphate, 1.25% ammonium sulfate, 0.45% magnesium sulfate, 0.04% calcium sulfate by optimization of multiplication medium BMGY.
出处
《粮油食品科技》
北大核心
2014年第2期100-103,共4页
Science and Technology of Cereals,Oils and Foods
基金
国家重点基础研究发展计划(2013CB127805)
科研院所技术开发研究专项资金(2012)
