SephadexLH-20纯化红松松球鳞片多酚及其体外抗氧化研究

Purification of polyphenols from Korean pine cone lamella by SephadexLH-20 and their antioxidative activities in vitro

为了得到高纯度红松松球鳞片多酚样品和初步评价其抗氧化活性,采用葡萄糖凝胶LH-20对红松松球鳞片多酚的初级纯化物进行二次精制,得到了纯度为95.86%的多酚样品。该多酚样品对DPPH自由基、ABTS自由基和羟自由基具有良好的清除作用,IC50分别为20.48、86.59μg/mL和0.45mg/mL;对铁离子(Fe3+)和铜离子(Cu2+)具有很强的还原能力,其还原铁离子(Fe3+)能力与V C相近,还原铜离子(Cu2+)能力略低于V C;对亚铁离子具有一定的螯合作用,其IC50为2.14mg/mL;对卵黄脂质过氧化具有明显的抑制作用,其IC50为0.33mg/mL。

A polyphenols-rich extract from Korean pine cone lamella after the preliminary purification was further purified by Sephadex LH-20 gel chromatography, and high purified polyphenol （HPP）which purity reached 95.86% was obtained. In order to estimate the capacity of HPP to act as antioxidants, its DPPH radical, ABTS radical, hydroxyl radical scavenging activity, ferric ions（ Fe3 ＋ ） and cupric ions （ Cu2 ＋ ） reducing ability, ferrous ions （Fe2＋） chelating activity, yelk peroxidation inhibiting effect were studied. HPP was found to exhibit remarkable scavenging activity on DPPH. （ IC50 20.48μg/mL）, ABTS ＋ （ IC50 86.59μg/mL）, OH. （ IC50 0.45mg/mL）, as well as a high and dose- dependent Fe3＋ Cu2＋ reducing power. Moreover, it possessed good chelating activity （IC50 2A4mg/mL） and yelk peroxidation inhibiting effect（ IC50 0.33mg/mL）.