定向驯化筛选耐毒酵母

Adaptation of a Saccharomyces Cerevisiae strain to lignocellulosic inhibitors by domestication

通过向五种驯化培养基中逐渐增加抑制剂,经过23d的连续驯化后,找到一株适用于纤维素水解液发酵生产乙醇的耐毒酵母。耐毒酵母和原始菌株在含有乙酸3.2g/L,糠醛0.8g/L,甲酸0.4g/L的培养基中生长时,耐毒酵母的乙醇产率为0.428g/g,达到理论值的85.6%,而原始菌株产率为0.246g/g,仅达到理论值的52.8%。耐毒酵母经连续传代5次,其产乙醇性能基本保持稳定。与原始菌株相比,耐毒酵母展现了良好的耐毒性状和高效利用葡萄糖发酵产生乙醇的能力。

In order to find the strains which could reach high ethanol production as well as telerate inhibitors on the lignocellulose acid hydrolysates.Through sequentially increasing in the concentration of inhibitory compounds in five different acclimation media and acclimatizing 23 days, the yeast resistant to drug was obtained suitable to produce the ethanol from the lignocellulose acid hydrolyates .When the yeast resistant to drug and the parent strain grew in the same media which contained several inhibitory compounds 3.2g/L acetic acid,0.Sg/L furfural,0.4g/L formic acid,the drug resistant yeast＇ s maximal ethanol yield could reach 0.428g/g, up to 85.6% of theoretical ethanol yield.Compared with drug resistant yeast, the parent strain＇s maximal ethanol production yield only could reach 0.246g/g, up to 52.8% of theoretical ethanol yield .After 5 continuous passages, the average ability of producing ethanol was stable.Compared with parent strain ,the yeast resistant to drug had good ability to ferment glucose and produce ethanol as well as tolerate inhibitors.