响应面法优化从石榴皮制备鞣花酸的工艺研究

Optimization of acidification process conditions for preparing ellagic acid from pomegranate peel by response surface methodology

以石榴皮为原料,采用响应面法研究鞣花酸酸化工艺条件。以多酚提取物转化率为指标,考察硫酸浓度、酸化时间、酸化温度对鞣花酸得率的影响。在单因素实验的基础上,通过Box-Benhnken中心组合设计和响应面分析法,确定石榴皮鞣花酸的酸化工艺条件。结果表明,优化的鞣花酸酸化工艺为:硫酸浓度为0.83mol/L、酸化时间5h、酸化温度105℃,石榴皮多酚提取物转化率为86.38%,用无水甲醇热回流萃取4次,得到鞣花酸样品。建立了石榴皮酸化制备鞣花酸的二次多项数学模型,对石榴皮鞣花酸的制备工艺具有较好的预测作用。

This paper examines the optimum acidification process conditions for preparing ellagic acid from pomegranate peel by response surface methodology.The effects of the concentration of sulfuric acid, acidification time and temperature on the yield of ellagic acid were studied based on polyphenols extract conversion rate. According to the single factor experiments, the optimum acidification process of ellagic acid was determined through Box-Benhnken central composite design and response surface methodology.The results showed that when the pomegranate peel polyphenols extract conversion rate reached up to 86.38% ,the acidification process conditions,i.e, the optimal ones were as follows： sulfuric acid of 0.83mol/L, the acidification time of 5h and acidification temperature at 105℃.And ellagic acid samples were obtained with anhydrous methanol heat reflux extraction of 4 times.A quadratic equation model for the preparation of ellagic acid by the pomegranate peel polyphenols extract acidification was set up and it played a good predicted role.