金荞麦水提物对COPD大鼠肺纤维化的影响

Influence of Fagopyrum Dibotrys Water Extract on COPD Model of Rats with Pulmonary Fibrosis

目的观察金荞麦水提物对慢性阻塞性肺疾病(COPD)大鼠模型肺纤维化的影响。方法改良烟熏法复制COPD模型,分正常组、模型组、金荞麦水提物低剂量组、金荞麦水提物高剂量组4组,观察支气管和肺组织病理,免疫组织化学检测基质金属蛋白酶(MMP-9)、基质金属蛋白酶组织抑制因子(TIMP-1),放射免疫法血清上清液检测转化生长因子(TGF-β)、Ⅲ型前胶原肽(PⅢ)。结果与模型组比较,金荞麦水提物组能显著减轻大鼠支气管、肺组织的病理变化,以高剂量最为明显,抑制MMP-9和TIMP-1的过度表达,升高MMP-9/TIMP-1,降低血清TGF-β和PⅢ的水平。结论金荞麦水提物能改善COPD大鼠模型的肺纤维化。

Objective：To study the effect of the water extract of Fagopyrum dibotrys on COPD model of rats with pulmonary fibrosis.Methods：Rats were randomly divided into normal group,model group,and Fagopyrum dibotrys water-extraction low and high dose groups.Pathological morphological changes of lung and bronchus were observed.The expressions of MMP-9 and TIMP-1 were detected by immunohistochemistry method.The expressions of TGF-β and P Ⅲ were detected in serum supernatant by radioimmunoassay method.Results：Compared with normal group,Fagopyrum dibotrys water extract had significant improvement effect on the lung and bronchus pathomorphism changes of rats,especially in high dose group and inhibited excessive expressions of MMP-9/TIMP-1,increased the expression of MMP-9/TIMP-1,and decreased the expression of TGF-β and PⅢ in serum.Conclusion：Fagopyrum dibotrys water extract can improve the COPD model of rats with pulmonary fibrosis.