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南京地区汉族人群编码HNA-3抗原的SLC44A2基因多态性研究

Genetype frequencies of SLC44A2 encoding human neutrophil antigen-3 in Nanjing Han population,China
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摘要 目的探讨南京地区汉族人群中编码人类中性粒细胞抗原-3(human neutrophil antigen-3,HNA-3)的SLC44A2基因的多态性及等位基因频率分布。方法对南京地区107位随机献血者的SLC44A2基因采用聚合酶链反应(polymerase chain reaction,PCR)方法扩增其相关外显子及侧翼内含子,并进行测序分析。结果 HNA-3a/3a有46例,HNA-3a/3b 47例,HNA-3b/3b 14例,实验结果符合Hardy-Weinberg遗传定律。HNA-3a和HNA-3b在南京地区的频率分别为0.649 5和0.350 5。结论南京地区汉族人群HNA-3具有多态性,此多态性可能造成的抗-HNA-3a相关风险在临床上应引起重视。 Objective To investigate the polymorphism and frequency distribution of SLC44A2 alleles encoding HNA-3 antigen in Nanjing Han population. Methods 107 random blood donors＇ SLC44A2 relevant exons and adjacent introns were amplified by PCR method and sequencing analysis. Results 46 cases HNA-3a/3a,47 cases HNA-3a/3b and 14 ca- ses HNA-3b/3b were found and all statistics were consistent with the Hardy-Weinberg equilibrium law. The frequency dis- tribution of HNA-3a and HNA-3h was 0. 6495 and 0.3505 in Nanjing Han population, respectively. Conclusion The dis- tribution of HNA-3 is polymorphie in Nanjing Han population. The clinical risk of anti-HNA-3a should bring to the atten- tion.
作者 肖建宇 黄成垠 陈青
机构地区 江苏省血液中心
出处 《中国输血杂志》 CAS CSCD 北大核心 2014年第2期148-150,共3页 Chinese Journal of Blood Transfusion
基金 江苏省"十二五"期间"科教兴卫工程"医学重点人才(RC2011088) 2010年度江苏省卫生国际交流支撑计划项目
关键词 人类中性粒细胞抗原-3 等位基因频率 多态性 南京汉族人群 human neutrophil antigen-3 allele frequency polymorphism Nanjing Han population
分类号 R457.11 [医药卫生—治疗学]
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参考文献13

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二级参考文献23

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中国输血杂志
2014年 第2期

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