铜抑制单细胞绿藻生长的毒性效应

Toxic effects of copper on inhibition of the growths of unicellular green algae.

运用评价化学品对藻类毒性的标准实验方法,根据实验结果采用机率单位法进行数据处理,分别得出铜抑制蛋白核小球藻(Chlorella pyrenoidosa),斜生栅藻(Scenedesmus obliquus),月形藻(Closterium lunula)生长的96h半效应浓度(96h-EC50)分别为67,51,202(g/L,并对各剂量反应方程进行X2检验,结果表明均符合精度要求,计算出的96h-EC50真实可靠.研究结果表明, 斜生栅藻对铜最敏感,而月形藻对铜有很强的抵抗能力.这3种藻类对铜敏感度由大到小的顺序为斜生栅藻、蛋白核小球藻、月形藻.不同藻种细胞的大小不同和藻细胞壁表面的含硫基团不同是导致藻类对铜敏感性不同的主要原因.

：A standard test method for evaluating the toxicity of chemicals to algae was applied; and 96h-EC50 of copper on inhibition of the growths of Chlorella pyrenoidosa, Scenedesmus obliquus, Closterium lunula were calculated as 67,51 and 202μg/L respectively using the method of probability unit for data treatment based on the test results. The results from the X2 test of each dose-response equations showed that 96h-EC50s calculated were all precise and credible. It was found that S. obliquus was most sensitive to copper but C. lunula had a strong resistibility to copper. The toxicities of copper on inhibition of the growths are different. The order of algae sensibility to copper was: S. obliquus>C. pyrenoidosa>C. lunula. The different volumes of cells of different species of algae and the different amounts of functional groups containing sulfur on algae surface were the main cause for the different sensitivity of algae to copper.