摘要
为获得含量较高的马兰草生物碱,对马兰草总生物碱的提取工艺条件进行了优化,探讨了乙醇浓度、温度、时间和料液比对总生物碱提取的影响,并研究了其对羟基自由基的清除效果。结果表明:马兰草总生物碱的最佳提取工艺为乙醇浓度40%,温度为50℃,料液比1∶25(g/mL),提取时间为75min。在该条件下的总生物碱含量为3.33mg/g,提取液对羟自由基具有一定的清除能力,当提取液生物碱浓度为0.040 0mg/mL时,对·O H的清除率达到42.08%。
To study on optimal conditions for extracting high concentration of total alkaloid from K.indical,the effects of ethanol concentration,temperature,time and solid-liquid ratio on exacting total alkaloid were explored and the cleanup effect on hydroxyl radical was studied either.The results showed that the optimum extraction conditions included 40% ethanol as extractant, temperature on 50℃, extraction time by 75 min,ratio of material to liquid on 1 ∶ 25 (g/mL).In this case,the total alkaloid could reach 3.33 mg/g and the extracting solution has certain antioxidative effect.When the concentration of total alkaloid was 0.040 0 mg/mL,the rate of scavenging hydroxyl radicals reached 42.08%.
出处
《贵州农业科学》
CAS
北大核心
2014年第2期162-164,共3页
Guizhou Agricultural Sciences
基金
泉州市科技计划项目"龙眼核药用成分的超声提取工艺及其生物活性的研究"(2007N4)
关键词
马兰草
总生物碱
抗氧化性
Kalimeris indical
total alkaloid
oxidative stability