刺楸种质资源遗传多样性的SRAP分析

SRAP analysis on genetic diversity of Kalopanax septemlobus

采用SRAP标记对来自山东徂徕山、泰山、崂山、蒙山的71份刺楸种质进行遗传多样性分析．结果表明：12对引物共扩增出110条带，其中102条显示多态性，多态位点百分率（PPL）为92．73％；71份刺楸种质的遗传相似系数（GS）0．573～0．975，平均0．822，说明各种质间具有较高的遗传变异；种群间遗传分化系数（GST）为0．1360，表明有13．6％的变异存在于种群间，86．4％的变异存在于种群内，种群内的遗传分化显著大于种群间的遗传分化．UPGMA聚类表明71份种质在相似系数为0．71时可以聚为3类，总体上来源相同的种质优先聚类，但也存在交叉聚类．

The genetic diversity of Kalopanax septemlobus from Shandong was investigated by using SRAP marker. The results showed that 110 bands were amplified by 12 pairs of informative and reliable primers,of which 102 were polymorphic, resulting in a polymorphic rate of 92. 730%. GS values of 71 K. septemlobus materials were 0. 573-0. 975,whose average was 0. 822. There was higher genetic diversity a- mong all the materials. The genetic differentiation coefficient in populations was 0. 136, there were vari- ances of 13.6% in populations, 86.4% genetic variation existed within populations. This data illustrated genetic variation within population was larger than that among populations. UPGMA cluster analysis showed that 71 materials were classified into 3 groups when the similarity coefficient was 0. 71. Overall, materials from the same source mostly clustered together, but cross clustering was also exist. Finally, we discussed the cause of the endangerment status K. septemlobus and proposed some management strategies for effectively protecting the species.