棕榈酸对肝癌HepG2细胞自噬和凋亡的影响

Effect of palmitic acid on autophagy and apoptosis of hepatocellular carcinoma cell line HepG2

目的探讨棕榈酸对肝癌HepG2细胞自噬和凋亡的影响。方法采用含浓度为800μmol/L棕榈酸的DMEM高糖培养基培养肝癌HepG2细胞24 h,向HepG2细胞转染绿色荧光蛋白(GFP)-LC3质粒,通过观察LC3Ⅱ绿色荧光斑点确定自噬发生情况。利用BafA1抑制自噬后,采用M30免疫荧光检测细胞凋亡情况。结果棕榈酸刺激24 h明显增加自噬及凋亡细胞数量。阻断自噬,棕榈酸刺激引起的HepG2细胞凋亡水平明显增加。结论棕榈酸可刺激自噬发生,该自噬对肝癌HepG2细胞有一定的保护作用。

Objective To investigate the effect of palmitic acid （PA） on the autophagy and apoptosis of hepatocellu- lar carcinoma cell line HepG2. Methods We cuhured the hepatocellular carcinoma cell line HepG2 in the high glucose medium with concentration of 800 μ mol/L palmitic acid DMEM for 24 h, and the HepG2 cells were transfected with GFP- LC3 plasmid. Through the observation of LC3II green fluorescent spots, we determined the occurrence of autophagy. After inhibition of autophagy by BafA1, the apoptosis was detected by M30 immunofluorescenee. Results Twenty-four hours palmitic acid stimulation significantly increased the number of autophagy and apoptosis. If the autophagy was inhibited, HepG2 cell apoptosis induced by the stimulation of palmitic acid increased significantly. Conclusion Palmitic acid can stimulate autophagy, and the autophagy has protective effect on HepG2 cells.