新城疫病毒M蛋白通过自身核定位信号介导进入宿主细胞核

Matrix protein of Newcastle disease virus localizes to the nuclei by its nuclear localization signal

通过生物信息学方法预测了新城疫病毒(NDV)基质蛋白(M)的细胞核定位信号(NLS),用Overlap-PCR方法缺失NLS基因,分别构建M和M-ΔNLS EGFP重组质粒,转染单层鸡胚成纤维细胞(DF-1),激光共聚焦观察了M蛋白亚细胞定位。结果表明:M蛋白能进入宿主细胞核中,缺失NLS基因序列之后M蛋白只分布于细胞质中。结论:新城疫病毒M蛋白可以通过其核定位信号进入宿主细胞核。

Based on bioinformatics method, the nuclear localization signal （NLS） of Newcastle disease virus （NDV） matrix protein （M） was predicted and deleted by an Overlap-PCR assay. Subsequently, the plasmids including the full-length M gene and M-△NLS EGFP gene were constructed, respectively, and were transfected into monolayer DF-1 cells growing on the glass slides. The subcellular localization of M protein was measured by a laser confoeal microscope. The results showed that the M protein entered into the nuclei with the help of NLS. However, M protein with the NLS deleted did not enter into the nuclei. Hence, the NLS is the key of matrix protein in nuclear entry.