复方大枣片增强免疫功能的实验研究

Experimental Study on Compound Jujube Tablets in Improving Immunity

目的:探讨复方大枣片对小鼠免疫力的影响。方法:将SPF级昆明种雌性小鼠200只随机分为5个实验组,每个实验组再随机分为低、中、高剂量[0.167、0.333、1.000 g/(kg·bw)]组及对照组4组,连续给药30天,分别进行迟发型变态反应实验、淋巴细胞转化实验,并检测抗体生成细胞、测定血清溶血素;同时进行小鼠碳廓清实验、小鼠腹腔巨噬细胞吞噬鸡红细胞实验、NK细胞活性实验,测定相关免疫学指标。结果:1.000 g/(kg·bw)剂量组能增加小鼠迟发型变态反应能力、淋巴细胞转化能力、抗体生成细胞数、血清半数溶血值,与对照组比较差异有统计学意义(P<0.05);0.333、1.000 g/(kg·bw)剂量组能增加小鼠NK细胞活性,与对照组比较差异具有统计学意义(P<0.05);3个剂量组对小鼠体质量、脾脏/体质量比值、胸腺/体质量比值、小鼠单核-巨噬细胞碳廓清能力、腹腔巨噬细胞吞噬鸡红细胞能力均无明显影响(P>0.05)。结论:复方大枣片具有增强小鼠免疫功能的作用。

Objective： To investigate the influence of compound jujube tablets on the immunity of the mice. Methods： All 200 mice were randomized into five experimental groups, and each group divided into low, moderate and high dosage groups [-0.167, 0.333, 1.000 g/（kg· bw） 1 and the control group, for 30 consecutive days, the contents of antibody-producing cells （APC） and serum hemolysin were measured by delayed allergic reaction and lymphocyte transformation test respectively; associated immune indexes were detected by mice carbon clearance capacity test, mice peritoneal macrophage to swallow chicken red blood cells and the activity of NK cells. Results： High dosage group at the concentrations of 1.000g/（kg·bw） could increase the ability of delayed allergic reaction and lymphocyte transformation, the numbers of APC and HC50, which showed significant difference compared with the control group （P〈0.05）; high and moderate dosage groups in the concentrations of 0.333 and 1.000g/（kg·bw） could enhance the activity of NK cells, the difference had statistical meaning compared with the control group （P〈0.05）; there was insignificant difference in mice weight, the ratio of spleen/body weight, the ratio of thymus glang/weight, mononuclear macrophage carbon clearance capacity and the ability of peritoneal macrophage to swallow chicken red blood cells among the treatment groups with different dosages （P〉0.05）. Conclusion： Compound jujube tablets could enhance the immunity of the mice.