帕金森病患者血清RANTES水平及外周血CD4^+CD45RO^+T淋巴细胞比例测定及意义

The Circulating Levels of RANTES and CD4^+CD45RO^+T Cells in Parkinson's Disease

目的检测帕金森病(PD)患者血清RANTES水平及外周血CD4+CD45RO+T淋巴细胞比例,探讨其变化原因、相关影响因素及其在PD免疫异常发病机制中的作用。方法采用生物素双抗体夹心酶联免疫吸附法对43例PD患者(PD组)、33例健康人(正常对照组)血清调节活化正常T细胞表达与分泌的趋化因子(RANTES)水平进行检测,同时采用流式细胞术检测外周血CD4+CD45RO+T淋巴细胞比例。采用PD统一评分量表(UPDRSⅢ)对PD患者进行病情评估。依据性别、年龄、病程、治疗状况对PD患者进行分组比较。结果与对照组比较,PD组血清RANTES水平、外周血CD4+CD45RO+T淋巴细胞比例均明显升高(P均<0.01),PD组血清RANTES水平与外周血CD4+CD45RO+T淋巴细胞比例呈正相关(r=0.53,P<0.01),与UPDRS评分亦呈正相关(r=0.74,P<0.001)。依据性别、年龄、病程、治疗状况对PD患者分组比较,各组间均无统计学差异(P均>0.05)。结论与正常对照比较,PD患者血清RANTES水平及外周血CD4+CD45RO+T淋巴细胞比例均明显增高,2者显著正相关。血清RANTES水平与PD患者UPDRS评分正相关。提示活化小胶质细胞及CD4+T淋巴细胞介导的特异性免疫反应在PD的发病、进展过程中起重要作用。

Objective To detect the circulating concentrations of RANTES and the percentage of CD4＋CD45RO＋T cells in Parkinson＇ s disease, and to investigate the causes of those changes in PD as well as the influencing factors of them. Methods The serum RANTES levels of 43 PD pa- tients and 33 healthy subjects with age and sex-matched were measured by enzyme-linked immunolsorbent assay （ELISA）. And the percentage of circulating CD4＋CD45RO＋T cells were detected by using two-color flow cytometry. The united Parkinson ＇ s disease rate scale part III score （UPDRS m ） were evaluated in all studied PD patients. And all PD patients were divided into several subgroups according to the different gender, age, dura- tion and treatment status. Results The mean serum RANFES levels and the percentage of CD4＋CD45RO＋T cells of PD group are significantly high- er than the healthy subjects group （both P 〈 0.01 ）, moreover, a strong and significant correlation between RANTES and UPDRS m score was ob- served in patients with PD（r= 0.74, P 〈 0.00）. The PD group were also divided into several different subgroups according to different the gender, age, duration and treatment status, however, there is no significant differences in all subgroups respectively （all P 〉 0.05 ）. There were positive corre- lations found between RANTES and the percentage of CD4＋CD45RO＋T cells observed in PD group （r = 0.53, P 〈 0.01 ）. Conclusion The PD group presented with significantly higher RANTES and the percentage of CD4＋CD45RO＋T cells levels. And the RANTES levels were strongly and significantly correlated with the PD clinical subjective evaluation.However, the gender, the age, the duration and the treatment status cannot interfere the circulating levels of both of them. In PD group, There were positive correlations between RANTES levels and the percentage of CD4＋CD45RO＋T cells. Our findings may suggest that activated miemglia and the adaptive immunity responses induced by CD4＋T lymphocytes may play an important role in the pathogenetic mechanisms of PD.