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产B类伏马菌素黑曲霉菌株基因水平鉴定研究 被引量：3

Genetic identification of B-type fumonisin-producing Aspergillus niger
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摘要 目的建立基于基因水平的产B类伏马菌素黑曲霉菌株的鉴定方法。方法通过聚合酶链反应(polymerase chain reaction,PCR)和反转录-PCR(reverse transcription-PCR,RT-PCR)对产B类伏马菌素黑曲霉基因簇中8个关键基因的DNA和mRNA提取方法、扩增条件等进行优化,并对其表达产物进行分析。结果检测的19株黑曲霉菌株均携带与B类伏马菌素合成相关的8个关键基因的DNA。其中与产毒量相关性较强的基因为fum6、fum14和fum19,但不同产毒水平黑曲霉菌株中8个关键基因的mRNA表达量各异。结论 DNA水平不足以鉴别黑曲霉菌株B类伏马菌素的产毒能力,需要结合mRNA表达水平综合分析。 Objective To develop a method for identification of B-type fumonisin-producing Aspergillus nigerat genetic level. Methods The DNA and mRNA extraction method, conditions fo ramplification of eight key genes of B-type fumonisin-producing gene cluster of Aspergillus niger by polymerase chain reaction （PCR） and reverse transcription-PCR （RT-PCR） were optimized. The expression products were analyzed. Results The DNA of eight key genes related to B-type fumonisin biosynthesis were detected in all 19 strains of Aspergillus niger, fum6, fum14, fi^ml9 were the most predominant genes in Aspergillus niger in association with fumonisin production, but the mRNA expression level of the 8 key genes were different which leaded to different B-type fumonisin produce ability. Conclusion B-type and non-B-type fumonisin-producing Aspergillus nigercould not be distinguished at DNA level. DNA in combination with mRNA expression level was needed to identify B-type fumonisin-producing Aspergillus niger.
作者 韩小敏 张靖 韩春卉 赵熙 张宏元 江涛 李凤琴
机构地区 国家食品安全风险评估中心卫生部食品安全风险评估重点实验室
出处 《中国食品卫生杂志》 北大核心 2014年第2期105-110,共6页 Chinese Journal of Food Hygiene
基金 国家自然科学基金(81072307) 国家自然科学基金(31301489)
关键词 黑曲霉 B类伏马菌素 产毒基因 菌株鉴定 霉菌毒素 食品安全 Aspergillus niger B-type fumonisin toxin producing gene strain identification mycotoxin food safety
分类号 R155 [医药卫生—营养与食品卫生学] Q7 [生物学—分子生物学]
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参考文献22

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二级参考文献9

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中国食品卫生杂志
2014年 第2期

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