内蒙古牧区乳酸杆菌的分离及其药敏性检测

Isolation and antibiotic susceptibility of Lactic acid bacteria from pastoral areas in Inner Mongolia

以内蒙古牧区自然发酵的乳及乳制品中分离的18株乳酸杆菌为研究对象,首先通过菌株形态学和生理生化鉴定,然后采用二倍稀释法,测定了目前临床常用的3种氟喹诺酮类药物(左氧氟沙星、诺氟沙星和环丙沙星)对这18株乳酸杆菌的药物最低抑菌浓度(MIC),结果表明,这18株试验菌株均属于乳酸杆菌属,且这18株乳酸杆菌中有16株乳酸杆菌对这3种氟喹诺酮类药物均表现为不同程度的耐药,耐药率高达82.8%,有1株表现为敏感,1株对三种氟喹诺酮类药物分别表现为敏感、耐药和中介。

The test is based on 18 strains of Lactic acid bacteria separated from milk and dairy products in pastoral areas Inner Mongolia. Firstly, their characteristics of morphological physiological and biochemical were studied. And then used the double dilution method to determinate the bacteria＇s＇ minimum bacteriostasis concentration （MIC） on three kinds of Fluoroquinolone drugs （Levofloxacin,Norfloxacin and Ciprofloxacin） which are widely used in current clinical . The results indicate that all of 18 tested strains belong to the Lactobacillus. Among the 18 strains Lactic add bacteria there are 16 strains show different degrees of drug resistance to the 3 kinds of Fluoroquinolone .And the highest rate is 82.8%.As for the other strains, one of them perform sensitive, the other one respectively perform sensitive, resistance and intermediary of the 3 species Fhioroquinolone drugs.