摘要
目的:了解趋化因子配体18(CCL18)蛋白在卵巢癌组组织上皮细胞中的定位及定量.方法:使用激光捕获纤维切割(LCM)获取卵巢癌、卵巢良性肿瘤及卵巢正常组织的上皮细胞,免疫磁珠分别获取CCL18蛋白,微量层析柱浓缩CCL18样品,并除盐后,基质辅助激光解吸电离飞行时间-质谱技术(MALDI-TOF)检测CCL18蛋白在不同组织细胞中的表达情况.结果:卵巢癌组织上皮细胞中CCL18蛋白呈高表达,且阳性率为9/10例,表达量为103~105;卵巢良性肿瘤组织上皮细胞中CCL18蛋白阳性率为2/6例,表达量为10a~104,而卵巢正常组织上皮细胞中未检测到CCL18蛋白的表达.结论:CCL18蛋白在卵巢癌组织上皮细胞中明显高表达,可能成为卵巢癌的新一代肿瘤标志物.
Objective:To explore the expression of CCL18 protein in different epithelial cells of ovarian cancer,benign ovarian tumor and normal ovarian organ.Methods:The epithelial cells of ovarian cancer,benign ovarian tumor and normal ovarian organ were captured with laser capture microdissection (LCM),CCL18 protein in epithelial cells was gotten with immunomagnetic beads,concentrated by trace column,desalinated,and then the expression of CCL18 protein was detected with matrix-assisted laser desorption ionization time of flight-mass spectrometry (MALDI-TOF).Results:CCL18 protein was high expressed in epithelial cells of ovarian cancer,the positive rate was 9/10 cases.The positive rate was 2/6 cases in epithelial cells of benign ovarian tumor.No CCL18 protein expression was detected in normal ovarian epithelial cells.Conclusion:CCL18 protein expression is high in malignant ovarian epithelial cells,suggesting that CCL18 protein is a potential tumor maker of ovarian cancer.
出处
《广西医科大学学报》
CAS
2014年第1期9-13,共5页
Journal of Guangxi Medical University
基金
广西自然科学基金资助项目(No.2011GXNSFA018190)