中药三七提取液对HepG2细胞侵袭转移性的影响

EFFECT OF HERBOFSANQI EXTRACT ON HepG2 CELLS INVASIONANDMETASIS

目的:研究三七提取液体外对人肝癌HepG2细胞株血管生成及其诱导血管内皮细胞迁移的影响。方法:将HepG2细胞分为不同浓度三七提取液处理组及对照组,采用MTT法观察三七处理液对HepG2细胞的抑制率;共培养法(Marigel invasion chamber),观察不同浓度的三七处理液诱导血管内皮细胞迁移的抑制作用,ELISA检测细胞上清液中Ang-1、Ang-2、VEGF、HIF-1α的水平。结果:三七处理液浓度达25mg/L时,对其诱导人脐静脉内皮细胞迁移开始表现出抑制作用,24,48,72h抑制率分别达3.57%、11.29%、13.16%,浓度达800mg/L时,抑制率达64.85%、67.74%、85.53%;经三七提取液处理后HepG2细胞迁移数为(220±2.30)个,抑制率达(63.67±3.32)%,细胞迁移数与阳性对照组(614±3.32)个比较,差异有统计学意义(P<0.01)。Ang-1、Ang-2、VEGF、HIF-1α水平与对照组比较,分别为(17.13±2.86)μg/L vs(32.54±6.82)μg/L、(332.28±64.22)μg/L vs(202±28.22)μg/L、(4.02±0.58)μg/L vs(2.56±0.25)μg/L、(258.74±123.56)μg/L vs(90.32±36.66)μg/L,差异有统计学意义(P<0.05)。结论:三七提取液对HepG2细胞增殖具有抑制作用,且能诱导血管内皮细胞迁移,对Ang-1、Ang-2、VEGF、HIF-1α也具有抑制作用。

Objective：To investigate the effect of herbSanQi on proliferation,tumor angiogenesis and endothelial cell migration of HepG2 human hepatocarcinoma cell in vitro.Methods：HepG2 cells were divided into SanQi treatment and control groups,the effect of herbSanQi on HepG2 human hepatocarcinoma cell was determined by MTT assay.Marigel invasion chamber was used to observe the different concentrations of SanQi induced endothelial cell migration inhibitory effect.ELISA assay was used to detect Ang-1,Ang2,Tie-2,HIF-1α of tumor cells supernatant after herbSanQi treatment.Results：When SanQi concentration rose to 25 mg/L,the induced human umbilical vein endothelial cell migration effect became inhibitory effect,the inhibition rate of 24,48 and 72 h was 3.57％,11.29％ and 13.16％,respectively,when the concentration rose to 800 mg/L,the inhibition rate was 64.85％,67.74％ and 85.53％,respectively.Compared with control group,the HepG2 cell migration number in SanQi treatment group was （614± 3.32） vs.（220±2.30）,the inhibition rate was （63.67±3.32）％.The levels of Ang-1,Ang-2,VEGF and HIF-1α were （17.13±2.86） vs.（32.54±6.82） μg/L,（332.28±64.22） vs.（202±28.22） μg/L,（4.02 ±0.58） vs.（2.56±0.25） μg/L,and （258.74±123.56） vs.（90.32±36.66） μg/L,respectively.The difference was statistically significant （P ＜0.05）.Conclusion：The herbSanQi extract can inhibit HepG2 cell proliferation,and induce endothelial cell migration.Ang-1,Ang-2,VEGF,and HIF-1 alpha can also be inhibited.