时间因素对PAMAM诱导牙本质再矿化的影响

TIME FACTORS IN REMINERALIZATION OF DENTIN INDUCED BY PAMAM

目的:探讨不同时间羧基改性的聚酰胺-胺型(PAMAM)对牙本质再矿化的影响。方法:选取20个牙本质块,随机分成A、B、C、D、E组,经37%的磷酸凝胶酸蚀后,A组作为空白对照不做任何处理,B^E组放置于羧基改性的PAMAM溶液中处理20min,再放置于饱和Ca(OH)2溶液中预处理30min,然后分别浸泡在人工唾液中矿化1,3,5,7d。利用扫描电镜(SEM)评估牙本质再矿化的效果。结果:SEM显示各组牙本质小管的矿化程度不同,随矿化时间延长,小管内矿化物逐渐形成,矿化物与牙本质小管的结合逐渐紧密。结论:PAMAM诱导牙本质形成的矿化物随着时间的增加而增加,提示了PAMAM在牙本质敏感的治疗方面有一定的潜在应用价值。

Objective：To evaluate the effect of carboxyl-modified polyamidoamine dendrimers （PAMAM） on the reminer alization of human dentin in different time.Methods：Twenty dentin discs were randomly divided into groups A,B,C,D and E.After demineralized with 37％ phosphoric acid,group A was served as the control,and groups B to E were first treated with carboxyl-modified PAMAM for 20 minutes,and then pretreatment with satu-rated Ca（OH）2 solution for 30 minutes before immersing into artificial saliva for a period of time （1,3,5 and 7 d） respectively.The dentin discs were characterized by scanning electron microscope （SEM）.Results：SEM showed that the results were different in different groups.The minerals in dentinal tubules were formed gradually and the combination of them close gradually with the time.Conclusion：The experimental results showed that polyamidoamine dendrimers induce remineralization of dentin obviously with the time.Consequently,this strategy is valuable for developing a new method to treat dentin hype-rsensitivity.