人肺癌细胞LALU血管生成过程的形态学观察及意义

Histopathological observation of the angiogenesis process induced by human lung cancer cell LALU and its significance

目的 :观察人肺癌细胞血管生成过程的病理形态学、超微结构特点及意义。方法 :采用人肺癌细胞LALU皮下移植瘤模型 ,以病理动态形态学及透射电镜观察不同时期肿瘤血管生成状态。结果 :光镜显示 ,人肺癌移植瘤第 2天至第 10天之间可分为血管生成前期和血管形成期 ,在肿瘤血管生成的第 2 0天出现肺转移灶。电镜显示 ,人肺癌移植瘤第 2天出现成血管细胞 ,第 4～ 10天 ,不成熟血管内皮细胞逐渐形成血管腔伴有较完整的新生基底板 ,内皮细胞趋向成熟发展 ,第 2 0天肿瘤血管内皮细胞更成熟 ,部分区域新生毛细血管基底板发育不全或缺陷。在以上全过程中癌细胞突起直接与成血管细胞、血管内皮细胞及血管壁相连。结论 :人肺癌细胞可诱导自身肿瘤血管生成 ,病理形态学及电镜有其特征性形态学改变 ,且与转移密切相关 。

Purpose To observe histopathological and ultrastructural characteristics of angiogenesis process. Method Human lung cancer cell,LALU was implanted into subcutis on SCID mice and angiogenesis process was continuously observed with light and electron microscopes. Results Light microscope observation showed that the angiogenesis process of transplantating tumor was divided into the protophase of tumor angiogenesis and stage of angiogenesis from the second day to the tenth day. At the 20th day, the metastasis tumor was found in the lung. Electron microscopic observation showed that at the 2nd day after tumor transplantating angioblast cells were present. During the 4th day to the 10th day, vessel endothelial cells were forming blood vessel, and basement membrane was uncomplete. The cancer cells were connected with angioblast cells, vessel endothelial cell and blood vessel wall during the angiogenesis process. Conclusions The human lung cancer may induce itself angiogenesis. The morphological change is observed by histopatholgy and ultrastructural study, and is correlated with metastasis. The feature of tumor angiogenesis process may be used as an important indicator for treatment of lung cancer.