鸭乙型肝炎肝纤维化模型的研究

Duck model of hepatic fibrosis induced by duck hepatitis B virus

目的 :用鸭乙型肝炎病毒阳性血清反复攻击建立鸭乙型肝炎肝纤维化动物模型。方法 :用鸭乙型肝炎病毒阳性血清0 1ml/只 (含DHBV颗粒 0 5× 10 9)从胫静脉注射 1日龄樱桃谷鸭 ,每周 1次 ,从每 10周剂量加大为 0 2ml/只 (含DHBV颗粒 2 5× 10 9) ,16周末处死动物 ,观察肝纤维化指标及肝脏组织病理学变化。结果 :用此方法诱发鸭肝纤维化形成率达87 5 % ,与四氯化碳腹腔注射诱发肝纤维化形成率接近 ,肝纤维化指标羟脯氨酸、透明质酸和Ⅲ型前胶原明显增高 ,与肝纤维化程度一致。结论

Purpose To study duck model of hepatic fibrosis induced by duck hepatitis B virus(DHBV). Methods Every duck born within 24 hours was injected with 0 1 ml (DHBV 0 5×10 9) from tibia vein once a week. From the tenth week,the dosage of everyone was increased to 0 2 ml(DHBV 2 5×10 9). Duck was killed after the sixteenth week. The indexes of hepatic fibrosis were assayed and the histopathologic changes were observed. Results The rate of duck hepatic fibrosis was 87 5%,which showed no difference with the model induced by CCl 4. The indexes of Hyp,HA and PCⅢ were increased significantly. Conclusion Duck model of hepatic fibrosis is successfully induced by DHBV was success.