p16基因在人胃癌活检标本中表达的意义

SIGNIFICANCE OF THE EXPRESSION OF p16 GENE IN BIOPSY SAMPLES FROM GASTRIC CANCER

目的 从基因水平上研究胃癌患者活检标本p1 6基因表达及临床意义。方法 应用PCR SSCP SILVERSTAINING方法 ,检测活检标本 p1 6基因表达情况。 结果 40例胃癌活检标本中 ,p1 6基因外显子 2表达异常例数为 1 9(47.5 % )。而在 2 8例慢性胃炎活检标本中 ,p1 6基因外显子 2表达异常例数为 2 (7.1 4 % )。且这两例均伴有重度肠上皮化生。结论 这些资料提示慢性胃炎并非癌前疾病 ,不存在 p1 6基因异常表达。但当合并有重度肠上皮化生时 ,可能存在抑癌基因表达异常情况。

Objective To study the clinical significance of the expression of p16 gene in biopsy sample from gastric cancer at gene level.Methods PCR-SSCP silver staining method was applied to the test of the expression of p16 gene in biopsy samples.Results Out of 40 specimens of gastric cancer, exon 2 expression of p16 gene was found abnormal in 19 cases ( 47.5 %), while out of 28 specimens of chronic gastritis, exon 2 expression of p16 gene was detected to be abnormal in only 2 cases (7.14%), which were accompanied with dysplasia.Conclusion These data suggest that chronic gastritis is not pre-cancerous disease, and when dysplasia occurred abnormality of tumor suppressor gene may exist.