梅花鹿FGF10基因的生物信息学分析

Bioinformatics analysis of on FGF10 in Cervus nippon

应用生物信息学的方法对梅花鹿FGF10基因的核苷酸和氨基酸序列进行了初步的生物信息学分析,包括理化性质分析、信号肽和跨膜结构域分析、磷酸化位点和疏水性分析、蛋白质二级结构分析、功能结构域分析以及系统进化分析.结果表明:梅花鹿FGF10基因编码213个氨基酸,蛋白相对分子量为23.84kD,为碱性不稳定蛋白;存在信号肽和跨膜结构域;共有26个磷酸化位点;二级结构主要由α螺旋、β转角、延伸链和随机卷曲组成.具有FGF典型的FGF结构域.系统进化分析显示,梅花鹿FGF10与哺乳动物FGF10相似性较高,并且与牛、羊在亲缘关系上最相近.为梅花鹿FGF10基因的结构和功能的进一步研究打下了坚实的理论基础.

We use the bioinformatics approach to analyse the nucleotide and amino acid sequence of FGF10 of Cervus nippon ,including as the character of physical and chemical ,the sigal peptide and transmembrane domain prediction ,analysis of phosphorylation sites and hydrophobicity ,secondary structure prediction domains ,prediction of the conserved domains and phylogenetic analysis .The re-sults showed that FGF10 of Cervus nippon encode a deduced 213 amino acid ,had a predicted molecu-lar weight of 23 .84 kD ,and the protein which had sigal peptide ,transmembrane domain and 26 phosphorylation sites is alkaline and instability .Helix secondary structure mainly composed of αhe-lix ,βturn ,extended strand and random crimped .And it also had a special FGF domain .Phyloge-netic analysis showed that FGF10 of deer had a very high homology with mammals ,and with cattle and sheep on the closest genetic relationship .These studies built a theoretical foundation for the fur-ther research on the structure and function of FG F10 .