新型铁系金属(M=Ni,Co)配合物的合成及其与DNA的相互作用

Synthesis of Two Novel Metal( M = Ni,Co) Complexes and Their Interaction with DNA

以1,10-邻菲罗啉(Phen)和2,4-二氨基-6-(2-吡嗪)-均三嗪(DAPT)为配体,与铁系金属(M=Ni,Co)盐反应合成了两个新型的金属配合物———[Ni(Phen)(DAPT)Cl2]·3H2O(3a)和[Co(Phen)(DAPT)Cl2]·3H2O(3b),其结构和性能经IR,LC-MS,元素分析,TG-DTA和摩尔电导率表征。用UV-Vis、溴化乙锭荧光竞争光谱、相对粘度、电化学、DNA热变性及盐效应等方法研究了3a和3b与小牛胸腺DNA(ct-DNA)的相互作用。实验结果表明:3a和3b与ct-DNA的结合模式均为插入模式;3a与ct-DNA的结合强度略大于3b。

Two novel metal（M =Ni,Co） complexes,[Ni（Phen） （DAPT） Cl2] ·3H2O（3a） and [Co （Phen） （DAPT） Cl2]· 3H2O （3b）,were synthesized by coordination of 1,10-phenanthroline （Phen） with 2,4-diamido-6-（2-pyrazine）-1,3,5-triazine （DAPT） and Nickel （Ⅱ） or Cobalt （Ⅱ）.The structures and properties were characterized by IR,LC-MS,elemental analysis,TG-DTA and molar conductivity.The interaction of 3a,3b with calf thymus DNA （ct-DNA） were investigated by UVVis,EB competition FL,relative viscosity,cyclic voltammetry,DNA thermal denaturation and salt effects method.The results showed that the interaction models of 3a and 3b with ct-DNA were intercalative model; 3a exhibited stronger affinity force compared with 3b.