火鹤花微体快繁试验报告

Researches on Rapid Microculture of Anthutium Scherzerianum

对火鹤花微体快繁从外植体的建立、继代培养、生根培养到移栽方法,进行了一系列研究,初步探明了影响微体快繁效果的主要因素。结果表明,火鹤花外植体消毒以0.1%HgCl2液消毒10min;外植体培养基以MS+6-BA1mg/L+IBA0.5mg/L+蔗糖30g/L+琼脂5.5g/L,pH=6.1;继代培养基以MS+6-BA1.5～2.0mg/L+IBA0.5mg/L+蔗糖25～35g/L+琼脂6g/L,pH=6.1;生根培养基以1/2MS为佳;移栽基质以1/2MS培养基+珍珠岩为好;工厂化生产以细河砂:蛭石=2∶1为基质并浇豆饼水较为经济合理。

The establishment of explant,subculture,rooting and transplanting of Anthurium scherzerianum were studied extensivels.The main factors effecting microculture were discovered.The results showed the best methed of discontamination is 0.1% HgCl 2 solution for 10 minutes.The best ingredients are Ms+6-BA 1mg/L+IBA 0.5mg/L+sucrose 30g/L+agar 5.5g/L with pH=6.1 for explant cualture;MS+6-BA 1.5-2.0mg/L+IBA 0.5mg/L+sucrose 25-35g/L+agar 6g/L with pH=6.1 for subculture;1/2MS for rooting;1/2MS+pear/rockfor transplanting; fine river sands:vermiculite=2∶1 as the material for industrial production.