Isolation and Preliminary Identification of Broad-spectrum Bacteriocin-producing Lactobacillus Strain CW3

[ Objective] This study aimed to screen and preliminarily identify LactobaciUus strain CW3. [ Method] Oxford cup double-plate method was used for preliminary screening of broad-spectrum bacteriacin-producing LactobaciUus strain; after eliminating the interference of organic acid and hydrogen peroxide, antibac- terial substances containing protein properties produced by the screened strain were detected; finally, the obtained strain was identified. [ Result] After elimination of interference factors such as organic acid and hydrogen peroxide, the fermentation supernatant of strain CW3 could stil｜ inhibit the growth of indicator strains; the antibacterial activity of fermentation broth was significantly reduced after treatment with trypsase and pepsase, which indicated that the produced antibacterial sub- stances were bacteriocins. Results of physiological and biochemical identification showed that strain CW3 belonged to Lactobacillus plantarum. [ Conclusion] CW3 is a broad-spectrum bacteriocin-producing LactobaciUus plantarum strain.