胃癌组织中跨膜丝氨酸蛋白酶4的表达及其与上皮间质转化的关系

Expression and correlation of TMPRSS4 and epithelial-mesenchymal transition in gastric carcinoma

目的探讨跨膜丝氨酸蛋白酶4(TMPRSS4)在人胃癌组织中的表达与临床病理特征之间的关系及对判断患者预后的意义,进一步研究其与上皮间质转化(EMT)的关系。方法采用免疫组织化学方法(SABC法)同时检测100例胃腺癌标本及其癌旁组织中TMPRSS4、波形蛋白(Vimentin)、上皮型钙黏蛋白(E-cadherin)的表达。结果在胃癌组织中TMPRSS4、E-cadherin、Vimentin的阳性率分别为47%、45%、37%,其癌旁组织阳性率分别为9%、30%、4%。3种蛋白在肿瘤组织和对应癌旁组织中的表达率之间差异有统计学意义(P<0.05)。TMPRSS4蛋白的表达与胃癌患者淋巴结转移、TNM分期有关(P<0.05),而与患者的性别、年龄、肿瘤组织大小、分化程度、浸润深度无显著相关性(P>0.05)。TMPRSS4蛋白表达阳性患者的5年生存率显著低于其阴性表达的患者。TMPRSS4与E-cadherin蛋白表达呈负相关(rs=-0.207,P=0.038),与Vimentin蛋白表达呈正相关(rs=0.233,P=0.020)。结论 TMPRSS4蛋白在人胃癌组织中的表达与肿瘤的生物学特性密切相关,并对预测肿瘤的侵袭转移有一定的参考价值。TMPRSS4可能通过降低E-cadherin的表达诱导EMT的发生来促进胃癌细胞的侵袭和转移。

Objective To investigate the relationship between the expressions of TMPRSS4 and clinical pathologi-cal parameters in gastric carcinoma. To explore its significance in judging the prognosis of patients. To analyze the expression of TMPRSS4 in the epithelial-mesenchymal transition. Methods The expressions of TMPRSS4, E-cad-herin and Vimentin were detected by immunohistochemistry （ SABC） in 100 cases of gastric carcinoma specimens and corresponding adjacent normal tissue. Results In gastric carcinoma, TMPRSS4, E-cadherin and Vimentin positive rate was 47%,45%,37%. In adjacent tissues,their positive rate was 9%,30%,4%. The differences be-tween tumor tissues and adjacent tissues had statistical significance （ P〈0.05 ） . High expression of TMPRSS4 cor-related significantly with lymph node metastases and TNM stage （P〈0.05）, but not with gender, age, size, tumor histological type,depth of invasion （P〉0.05）. The 5-year survival rate of patients with high TMPRSS4 expression was significantly lower than that in patients with low expression. The high TMPRSS4 expression was significantly correlated in gastric carcinoma accompanied by low expression of E-cadherin （rs = -0.207,P=0.038） and high Vimentin expression （rs=0.233,P=0.020）. Conclusion The expression of TMPRSS4 is closely related to the biological characteristics in gastric carcinoma,detection the expression of TMPRSS4 is valuable in predicting tumor prognosis,invasion and metastasis. TMPRSS4 may promote invasion, metastasis of human gastric carcinoma through epithelial-mesenchymal transition by reduce the expession of E-cadherin.