去脾大鼠对肝纤维化组织中PTEN基因表达的影响

PTEN gene regulation in liver fibrosis of rats after splenectomy

目的研究去脾脏功能对大鼠肝纤维化组织中PTEN基因表达的影响。方法胆总管结扎及脾脏切除建立去脾脏功能肝纤维化模型。HE染色及天狼星红染色显示肝纤维化程度；蛋白印迹、实时定量PCR及SP法测定大鼠肝组织中PTEN、a-平滑肌肌动蛋白（a-SMA）的表达并检验其相关性。结果PTENmRNA表达及PTEN蛋白表达量脾脏切除组高于脾脏假手术组（P〈0．05）；a-SMA的蛋白表达量脾脏切除组低于脾脏假手术组（P〈0．05）；PTEN蛋白表达量与a—SMA的蛋白表达量呈显著负相关（r=-0．86，P〈0．05）。结论肝纤维化大鼠模型中，去脾脏功能可上调PTEN在肝纤维化组织中的表达，并减少a-SMA的分泌从而延缓肝硬化的发生。

Objective To inve stigate in vestigate the effects of splenectomy on the expression of the PTEN gene in liver fibrosis of rats induced by biliary tract obstruction. Methods The liver fibrosis model was induced by bile duct ligation. Rats were randomly divided into 3 groups. Group A had bile duct ligation ＋ splenectomy （ BDL ＋ SPL, 45 rats）, group B had bile duct ligation ＋ spleen sham operation （ BDL ＋ SSP, 45 rats）, and group C had sham bile duct ligation ＋ spleen sham operation （ SBDL ＋ SSP, 45 rats）. Liver tissue samples from each group were taken in weeks 1,3, and 5. HE and Sirius staining displayed the degree of liver fibrosis. Western-blot, real-time PCR, and immunohistochemistry SP measured the expression of a-smooth muscle actin （a-SMA） together with the expression of PTEN mRNA and PTEN protein. The relevance was also tested in this study. Results As time increased, liver fibrosis gradually occurred in group A and B, and the degree of liver fibrosis was more serious in group B than in group A. The expression volume of PTEN mRNA and PTEN protein in group A was higher than that in group B （ P 〈 0.05）, while the expression volume of a-SMA was the opposite （P 〈 0.05 ）. The expression volume of PTEN mRNA and PTEN protein were negatively correlated to a-SMA （ r = - 0. 86, P 〈 0.05 ）. Conclusion In the rat liver fibrosis model, splenectomy up-regulated the expression of the PTEN gene and reduced the secretion of a-SMA, thereby delaying the progression of liver fibrosis.