bFGF与IL-6／STAT3信号通路在胶质瘤细胞中的作用关系研究

A study about the relationship between bFGF and IL -6/STAT3 signaling pathway in glioma cells in vitro

目的 探讨碱性成纤维细胞生长因子（bfGf）与IL-6／STAT3信号通路在胶质瘤细胞中的作用关系。方法将U251细胞分为对照组、空载组和实验组，空载组和实验组按感染复数（multiplicity of infection，MOI）=100分别进行空载体腺病毒（Ad-GFP）和含有bFGF小分子干扰RNA的重组腺病毒（Ad—bFGF—siRNA）转染，通过Westernblot方法检测相关蛋白的表达，并应用ELISA方法检测细胞上清液中IL-6的水平变化。结果实验组细胞经转染Ad-bFGF-siRNA后，Westernblot结果显示，与对照组和空载组相比pSTAT3（TyrT05）和pSTAT3（Ser727）表达下降，呈时间依赖性，同时STAT3上游的激酶pERKl／2、pJAK2、Src及下游的效应蛋白CyclinDl、Bcl—xl表达均降低；ELISA法检测细胞上清液中IL-6的水平显示，实验组上清液中IL-6浓度与对照组和空载体组相比明显降低，差异均有统计学意义（P〈0．05）。结论腺病毒介导的bFGF小干扰RNA通过抑制IL-6的分泌，减少了胶质瘤U251细胞系的STAT3信号通路的活化，包括抑制上游的调节激酶JAK2、ERK及下游的效应分子CyclinDl和Bcl—xl的表达。

Objective To investigate the role of the relationship between basic fibroblast growth factor and IL - 6/STAT3 signaling pathway in glioma cells. Methods U251 cells were divided into the control group, mock group and experimental group. The mock and experimental groups were transfected with mock vector（ Ad - GFP） and the recombinant adenovirus carrying bFGF - siRNA （ Ad - bFGF - siRNA） respectively at a multiplicity of infection （MOI） of 100. The expression of related proteins was revealed by the method of Western blot, and the levels of IL - 6 in the supernatant of U251 cells were measured via the method of ELISA. Results After U251 cells were transfected with bFGF - siRNA, the results of Westem blot showed that compared with the control group and empty vector group pSTAT3 （TyrT05） and pSTAT3 （Ser727） expression decreased, which was time dependent, also the expression of STAT3 upstream kinase pERK1/2, pJAK2, Src and the downstream effector proteins CyclinD1, Bcl- xl were decreased; the IL- 6 level in U251 cell supernatant detection via ELISA showed in the experimental group IL -6 concentration decreased obviously, the differences were statistically significant （ P 〈 0.05 ）. Conclusions Adenovirns mediated small interfering RNA targeting bFGF could reduce the activation of STAT3 signaling pathway in glioma U251 cell line by suppression of IL- 6 secretion, as well as inhibit the expression of the upstream regulating kinase JAK2, ERK and downstream effector molecules CyclinD1 and Bcl -xl.