Has-miR-339-5p对裸小鼠人乳腺癌移植瘤形成及生长的影响

Influence of Has-miR-339-5p on the formation and growth of transplantation tumor of breast cancer in nude mice

目的建立miR-339-5p的稳定转染细胞系并探讨其在小鼠体内的成瘤性。方法用Lipofectamine 2000介导的转染法将含miR-339-5p片段的质粒转染人乳腺癌细胞株MDA-MB-231,以空载体作为阴性对照。并用G418作为筛选用药建立稳定转染细胞系。皮下原位接种癌细胞观察其对乳腺癌的形成及生长的影响。结果 RT-PCR技术检测稳定转染细胞系miR-339-5p miRNA的表达,与阴性对照组相比,其表达明显上调;接种miR-339-5p稳定转染细胞系组小鼠形成乳腺癌的时间明显迟于阴性对照组,且肿瘤生长较慢。结论 miR-339-5p可降低体内乳腺癌细胞的生长繁殖能力,有望成为乳腺癌治疗的潜在新靶点。

Purpose To establish miR-339-5p stable transfection cell line and to study their tumorigenicity in nude mice.Methods Lipofectamine 2000 methods containing miR-339-5p fragment of plasmid was transfected into human breast cancer cell line MDA-MB-231 and empty vector was used as a negative control.Stable transfected cell lines were established by G418 as a screening drug.Influence of Has-miR-339-5p on the formation and growth of breast was observed by subcutaneous inoculation of cancer cells in situ.Results Expression of Has-miR-339-5p of cell lines stably transfected with miR-339-5p miRNA expression was significantly increased compared with the negative control by RT-PCR.The formation time of breast cancer of miR-339-5p stably transfected cell lines group of mice was significantly later than that of negative control,and the tumors grew more slowly.Conclusion The miR-339-5p can reduce the growth and multiplication of breast cancer cells in vivo.It may be the potential candidate target for breast cancer treatment.