夏播菘蓝不同居群干物质和活性成分积累特征

Accumulating Characteristics of Dry Subtance and Active Component of Summer-planted Isatis indigotica Fort.Cultivated Populations

为研究夏播菘蓝不同栽培居群的最佳采收期，以来自于山西、安徽、甘肃、江苏和河南的5个栽培居群为材料，设置盆栽土培实验，于菘蓝生长第60天起，间隔10d采样，共采集6次样品，采用HPLC法测定其叶片、叶柄、根茎和根4个部位的靛蓝与靛玉红含量。结果表明：（1）不同栽培居群随着生长时间的延长，其叶片、叶柄、根茎及根的生物量持续增加，其中来自于山西居群的生长最佳，干物质积累量最大。（2）不同居群叶片及叶柄内靛蓝与靛玉红含量最大值出现在生长90～100d，即为叶内活性成分积累的高峰期。（3）综合分析其活性成分与干物质的积累量，各居群叶片靛蓝积累量最大值在生长90～100d，叶片、叶柄与根茎的靛玉红积累量也出现在90～100d。若以中国药典的靛玉红为质量控制指标，同时综合考察其生物量指标与活性成分积累量指标，夏季播种的来自于山西、安徽和河南的菘蓝居群最佳采收期为生长100d左右，而来自于甘肃与江苏居群的则以生长90d最佳。

To study the best harvesting time of summer-planted Isatis indigotica Fort. , we planted different cultivated populations, which were from Shanxi, Anhui,Gansu,Jiangsu and Henan Province, by pot culture experiment. Test samples, which were sampling interval 10 days since growing 60 days,were harvested for 6 times. The contents of indigo and indirubin in their leaves, petioles, rhizomes and roots were determined by HPLC. The results showed that. （1）The biomass of leaves, petioles, rhizomes and roots increased with the growth time, in which the growth was the best, and dry matter accumulation was the maximum is the population from Shanxi. （2）The highest contents of indigo and indirubin in leaves and petioles of different populations occurred 90～100 days,namely a peak period of active components in leaves. （3）The highest of indigo accumulating amount （dry weight multiplying indigo content） in leaves of every population of I. in- digotica appeared at 90～100 d,and the highest of indirubin accumulating amount in leaves,petiole and rhi- zome appeared at 90～100 d too. The most appropriate sampling time of I. indigotica from Shanxi,Anhui and Henan was 100 days,while the Gansu and Jiangsu populations were sampling at 90 days,taking indiru- bin as the quality control indicator in China Pharmacopoeia, meanwhile taking comprehensively the biomass and accumulating amount of active comDonent as comorehensive indicators.