一株具有脱胶功能大麻内生真菌的分离和鉴定

Isolation and Identification of an Endophytic Fungus for Microbial Degumming of Cannabis sativa L.

采用组织块法对大麻(Cannabis sativa)根、茎、叶等组织中的内生真菌进行分离,利用平板透明圈法筛选具有脱胶功能的菌株,对获得的脱胶菌菌株进行形态学鉴定和分子生物学鉴定。结果表明:(1)从大麻根、茎、叶的组织部位共分离得到内生真菌16株,茎部分离到9株真菌,叶部5株,根部2株。(2)编号为DM6的内生真菌具有较强的果胶分解能力,其透明圈直径为2.49cm。(3)形态学鉴定发现,内生真菌DM6不能产生孢子,菌丝较为粗壮、分支较少、有明显的隔;分子学鉴定表明,内生真菌DM6与Phoma aliena(KC311486)序列的相似性最高,为99%,并且在系统发育树上位于同一分支上。因而内生真菌DM6可以鉴定为茎点霉属一种(Phomasp.)。

Endophytic fungi were isolated from the roots, stems and leaves of Cannabis sativa L. by tissue block method,and degumming strains were screened by transparent circle method, and then identified by morphology and molecular biology methods. The results indicated that. （1）A total of 16 endophytic fungi were isolated from different tissues of the C. sativa,nine of them were from the stems, five then from the leaves and two from the roots （2）. （2）For the isolated strains,one of them named DM6 was more effective in decomposing the pectin of the C. sativa, and the transparent circle diameter is 2.49 cm. （3）Morphological identification showed that strain DM6 was non-sporulating and the hyphae was more stout, fewer branches with a distinct septum. Molecular identification indicated that the sequence of strain DM6 was found to share 99% similarity with Phoma aliena （KC311486）. Strain DM6 was shown to be close to the genus Pho- ma. So the isolate was further identified as Phoma sp.