有机肥发酵剂应用效果的研究Ⅰ.有机肥发酵剂菌种的筛选

Studies on the effect of the yeast for organic fertilizer PartⅠ Selection of the yeast strains for organic fertilizer

从黑龙江省佳木斯市四丰山林土样品、合江农科所内腐熟有机肥样品及日本产的 EM(Ef-fective Microorganisms)中分离筛选出 1 2个菌株 ,进行了碳源滤纸崩溃试验 ,鉴定出 4个高效菌株 ,并进行了形态特征观察。在不同培养基条件下进行了纤维素酶、蛋白酶、淀粉酶的酶活力测定 ,将上述 4个高效菌株配制成 6个组合 ,并分别测定了每个组合 3种酶活力 ,其中 I(F- 1× F- 4)和 (F- 1×F- 3)两个组合 3种酶的综合性能较好 , 和 两个组合中 F- 1、F- 3、F- 4三种菌株混合一起 ,作为有机肥发酵剂的菌种 。

Twelve strains were isoloted from samples of forest soil from Sifeng Mountain in Jiamusi city, samples of rotten organic fertilizer in Hejiang agricultural institute, and EM made in Japan. The experiment of the filter collapse was conducted, and 4 strains were determined. The morphological character of the strains was observed. The enzyme activity of cellulolytic enzyme,amylase and pro teinase were analyzed under different media. The 4 strains were arranged into 6 groups, and the ac tivities of 3 kinds of the enzyme from each group were analyzed, among them, the complex functions of the 3 kinds of enzyme activity ofⅠ(F-1×F-4) andⅡ(F-1×F-3) were very good. Both groupⅠand Ⅱwere composed of F-1,F-2,F-3 strains, and it was determined as the strains of the yeast for organic fertilizer. Technological process of the yeast for organic fertilizer had been developed.