碱性成纤维细胞生长因子胶原蛋白复合海绵作用于羟基磷灰石义眼台血管化过程的组织病理学观察

Effects of basic fibroblast growth factor composite sponge treated collagen on vascularization of orbital implants： a histopathologic analysis

目的将碱性成纤维细胞生长因子（bFGF）胶原蛋白复合海绵作用于羟基磷灰石（HA）义眼台，观察义眼台血管化的组织病理学改变。方法将bFGF胶原蛋白复合海绵包裹的HA义眼台植入实验兔眼窝，与植入无包裹的HA义眼台和胶原蛋白海绵包裹的HA义眼台进行对比，分别于植入后2、4、6、8、12周取出植人物，观察大体情况，并行光镜及电镜检查义眼台血管化改变，分析其对义眼台血管化的作用。结果在术后2、4、6周，bFGF胶原蛋白复合海绵组较其他两组血管化程度高，胶原蛋白组较无包裹组血管化程度高。bFGF胶原蛋白复合海绵组在术后6周义眼台完全血管化，其他两组在术后8周义眼台完全血管化。术后2、4、6周，各组之间HA义眼台纤维血管长度的平均值及纤维血管化程度级别差异有统计学意义（P〈0．05）；术后8、12周差异无统计学意义（P〉0．05）。结论bFGF胶原蛋白复合海绵能促进义眼台纤维血管化，缩短植入物血管化的时间，胶原蛋白海绵能促进早期的纤维血管化，但不能缩短完全血管化所需要的时间。

Objective To evaluate the effect of basic fibroblast growth factor （bFGF） treated collagen composite sponge on vascularization of HA orbital implants. Methods New Zealand rabbits received three different orbital implants：naked implants, implants wrapped with collagen composite sponge and implants wrapped with bFGF treated collagen composite sponge. Implants were harvested 2, 4, 6, 8 and 12 weeks after surgery. The vascularization of implants was then assessed by light and electron microscopy. Results At post-surgery weeks of 2, 4 and 6, bFGF trealed collagen composite sponge induced the highest degree of vaseularization of orbital implants. Collagen composite sponge alone resulted in higher extent of vaseularization than naked implants. Complete vascularlzation of implants was observed at post- surgery 6 weeks by bFGF treated collagen composite sponge, which was not observed in the other two groups until post-surgery 8 weeks. There were significant differences in the average length of fibrovasculature and in the degree of vascularization among each group at post-surgery 2, 4 and 6 weeks （ P 〈 0. 05 ） , while no statistical difference was observed at post-surgery 8 and 12 weeks （ P 〉 0. 05 ）. Conclusions bFGF treated collagen composite sponge facilitates fibrovascularization of orbital implants, and shortens the time required for complete vascularization. Collagen composite sponge alone promotes early-stage fibrovaseularization, but fails to facilitate complete vascularization of orbital implants.