CXCR2在胰腺导管细胞癌中的表达及意义

Significance and expression of CXCR2 in pancreatic ductal adenocarcinoma

目的:探讨CXC趋化因子受体2(CXCR2)在胰腺导管细胞癌中的表达以及与其发生、发展的关系。方法:采用RT-PCR法、Western blot免疫印迹法检测50例胰腺导管细胞癌组织及癌旁组织和30例正常胰腺组织中CXCR2的表达水平,分析其与胰腺导管细胞癌生物学特性的关系。结果:胰腺导管细胞癌组织、癌旁组织和正常胰腺组织中CXCR2 mRNA和蛋白的表达分别为1.13±0.25、0.79±0.18、0.78±0.14和1.75±0.21、1.01±0.15、0.96±0.20,同癌旁组织和正常胰腺组织比较,胰腺导管细胞腺癌组织中CXCR2 mRNA和蛋白的表达明显升高(P<0.05)。同正常胰腺组织相比,癌旁组织中CXCR2mRNA和蛋白表达无明显升高(P>0.05)。CXCR2 mRNA和蛋白表达与肿瘤分化程度、淋巴转移和TMN分期密切相关,差异有统计学意义(P<0.05)。结论:CXCR2在胰腺导管细胞癌中高表达,与胰腺导管细胞癌发生、迁移、侵袭和转移等恶性生物学行为相关。

Objective： To examine the expression of CXC chemokine receptor 2 （CXCR2） in pancreatic dutal adenocarcinoma, and determine its role in tumorigenesis and progression of pan- creatic dutal adenocarcinoma. Methods- CXCR2 mRNA and protein expression in 50 specimens of pancreatic dutal adenocarcinoma, and adjacent pancreatic tissues, 30 of normal pancreatic tissues was examined by using semi-quantitative reverse transcription-PCR and Western blot. Results：The expression levels of CXCR2 mRNA and protein in pancreatic dutal adenocarcinoma, adjacent pan- creatic tissues and normal pancreatic tissues were 1.13 ＋ 0.25,0.79 ＋ 0.18 and 0.78 ＋ 0.14 and 1.75 ＋ 0.21, 1.01 ＋ 0.15 and 0.96 ＋ 0.20 respectively. As compared with adjacent pancreatic tissues and normal pancreatic tissues, the expression levels of CXCR2 mRNA and protein in pancreatic dutal adenocarci- noma were significantly increased（P〈0.05）. No significant difference in the expression levels of CXCR2 mRNA and protein between adjacent pancreatic tissues and normal pancreatic tissues （P〉0.05）. The expression levels of CXCR2 mRNA and protein were relevant to the degree of tumor differentitation, lymphatic metastasis and TMN staging with the difference being significant（P〈O.05）. Conclusion： CXCR2 was highly expressed in pancreatic dutal adenocarcinoma. CXCR2 might play a role in the tu- morigenesis, migration, invasion and metastasis of pancreatic dutal adenocarcinoma.